| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rudd, Conner D (12)
|
ADCPP |
31.73 |
30.05 |
-1.68 |
| 2 |
Keller, Brady C (12)
|
ADCPP |
31.12 |
31.66 |
0.54 |
| 3 |
Killian, Daniel S (12)
|
ADCPP |
33.24 |
32.50 |
-0.74 |
| 4 |
Goodwill, Trevor M (12)
|
ADCPP |
32.30 |
32.73 |
0.43 |
| 5 |
Hook, Gavin M (12)
|
ADCPP |
34.90 |
33.86 |
-1.04 |
| 6 |
Tieckelmann, Connor R (12)
|
ADUN |
36.84 |
35.48 |
-1.36 |
| 7 |
McCoy, William G (12)
|
ADCPP |
36.36 |
37.10 |
0.74 |
| 8 |
Upton, Matthew T (12)
|
ADCPP |
37.98 |
37.98 |
0.00 |
| 9 |
Simmons, Cooper Z (12)
|
ADCPP |
36.46 |
38.00 |
1.54 |
| 10 |
Bhave, Advait G (12)
|
ADUN |
39.52 |
40.07 |
0.55 |
| 11 |
Ahn, Christopher Y (11)
|
ADCPP |
43.22 |
46.14 |
2.92 |
| 12 |
Nabaoui, Abdullah (11)
|
ADCPP |
48.58 |
49.97 |
1.39 |
| 13 |
Hughes Robillard, Eric M (11)
|
ADCPP |
55.00 |
56.34 |
1.34 |